河鲀小鼠结合蛋白.docx
- 苏小小个人认证 |
- 2021-04-18 发布|
- 109.6 KB|
- 15页
摘 要
本实验以无特定病原体级（SPF）ICR品系性雄性健康小鼠为实验对象。通过对小鼠灌胃法和腹腔注射TTX-重组蛋白混合液，研究重组蛋白对小鼠TTX中毒的解毒作用。结果表明灌胃剂量350 μg/kg BW时2h内小鼠死亡率为60%，与此作为对照组TTX浓度，灌胃毒素与蛋白孵化后混合液，所有小鼠均存活，表明TTX与蛋白体外孵育后，TTX呈无毒。而腹腔注射TTX浓度为7.5 μg/kg BW小鼠生存延长，腹腔注射毒素与蛋白孵化后混合液，小鼠存活率显著上升，表明TTX与蛋白体外孵育后，TTX的毒性显著降低。
关键词：TTX；河鲀；小鼠；TTX结合蛋白
Abstract
In this study, healthy male mice without specific pathogen grade (SPF) ICR strain were selected as subjects. The detoxification effect of recombinant protein on TTX intoxication in mice was studied by gavage and intraperitoneal injection of TTX- recombinant protein mixture. The results showed that the mortality rate of mice was 60% within 2h after gavage of 350 g/kg BW, which was used as the TTX concentration of the control group. All mice survived after gavage of the mixture of toxin and protein after incuba