2021年度食品科学与工程毕业论文.doc

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文档介绍

山东理工大学

毕业论文

题 目:动物组织中硝基呋喃代谢物残

留量测定办法

学 院:

专 业: 食品科学与工程

学生姓名:

指引教师:

毕业论文时间:二ОО八年二月二十五日~六月二十日 共十七周

摘 要

用高效液相色谱-串联质谱(LC-MS/MS)法同步测定动物组织及水产品中呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因代谢物。盐酸水解动物组织及水产品中蛋白结合代谢物,同步加入2-硝基苯甲醛(2-NBA),37℃

核心词:液相色谱-串联质谱,硝基呋喃类代谢物,内标法

Abstract

A high performance liquid chromatography-tandem mass spectrometric method was established for simultaneous determination of the nitrofuran metabolites,furazolidone,nitrofurazone and nitrofurantoin in animals,tissue and aquatic produces. Potein-bound metabolites were released in HCl solution and deriatised with 2-nitrobenzaldehyde(2-NBA)overnight at 37℃. Proteins were sedmentated by n-hexane ,after adjusting pH to 7.0 by sodium hydroxide. Then the analytes were extracted by ethyl acetate and cleaned up by n-hexane. Identification was achieved by electrosp

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