FPLCPPT:fplc.ppt

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FPLC All you need to know – in the available time! FPLC Fast Performance Liquid Chromatography- Liquid chromatography is a term which refers to all chromatographic methods in which the mobile phase is liquid. The stationary phase may be a liquid or a solid, in the form of a matrix A type of liquid chromatography where the solvent velocity is controlled by pumps. The pumps control the constant flow rate of the solvents FPLC The solvents are accessed through tubing from an outside reservoir. The flow rate of the solvent is set through computer input and controlled by pumps. There are various columns used in liquid chromatography depending on the type of separation preferred. Each column contains a small diameter packing material. The column is a large (mm id) tube containing small (u) partic

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